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USAscientific 1402-4700：八聯(lián)排管薄壁+無核酸酶，PCR實(shí)驗(yàn)新選擇

更新時(shí)間：2025-06-24 點(diǎn)擊次數(shù)：683

在分子生物學(xué)實(shí)驗(yàn)中，PCR（聚合酶鏈?zhǔn)椒磻?yīng)）是技術(shù)之一，而PCR耗材的穩(wěn)定性直接影響實(shí)驗(yàn)結(jié)果的可靠性。今天為大家介紹一款“細(xì)節(jié)控"實(shí)驗(yàn)室的寶藏耗材——USAscientific貨號(hào)1402-4700，0.2ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八聯(lián)排無核酸酶薄壁PCR管（以下簡稱“1402-4700八聯(lián)排管"）。它憑借多項(xiàng)創(chuàng)新設(shè)計(jì)，成為PCR實(shí)驗(yàn)的“效率加速器"。

產(chǎn)品詳細(xì)介紹：從結(jié)構(gòu)到材質(zhì)的“硬核配置"

1402-4700八聯(lián)排管是一款專為PCR實(shí)驗(yàn)優(yōu)化的耗材，核心設(shè)計(jì)圍繞“精準(zhǔn)、便捷、安全"展開：

- 品名：0.2 ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八聯(lián)排無核酸酶薄壁PCR管(0.2ml)

- 結(jié)構(gòu)設(shè)計(jì)：采用經(jīng)典8聯(lián)排條帶設(shè)計(jì)，每條包含8個(gè)獨(dú)立0.2ml PCR管，管體與平蓋通過“FLEX-FREE"彈性連接，既保證運(yùn)輸時(shí)的穩(wěn)定性，又支持單手輕松掰取單管，避免傳統(tǒng)連蓋管“暴力拆條"的麻煩。

- 材質(zhì)與工藝：管體采用醫(yī)療級(jí)聚丙烯（PP）材質(zhì)，經(jīng)伽馬射線輻照滅菌及無核酸酶處理工藝，確保無DNase/RNase殘留（第三方檢測報(bào)告驗(yàn)證）；透明平蓋采用相同材質(zhì)，與管體緊密貼合，耐高溫（-20℃~121℃），適配常規(guī)PCR熱循環(huán)儀。

- 參數(shù)規(guī)格：單管容量0.2ml（±0.5%誤差），管壁厚度僅0.15mm（薄壁設(shè)計(jì)），外徑12mm，高度47mm，符合主流PCR儀（如ABI 7500、Bio-Rad CFX系列等）的孔板適配要求。

核心優(yōu)勢：為什么它是PCR實(shí)驗(yàn)的“優(yōu)選"？

1. 無核酸酶污染風(fēng)險(xiǎn)：通過嚴(yán)格的去核酸酶工藝（輻照+化學(xué)處理），消除管壁殘留的核酸酶，降低實(shí)驗(yàn)假陽性概率，尤其適用于高靈敏度實(shí)驗(yàn)（如qPCR、痕量DNA擴(kuò)增）。

2. 薄壁設(shè)計(jì)，提升擴(kuò)增效率：0.15mm超薄管壁導(dǎo)熱更快，減少熱循環(huán)儀升降溫時(shí)間（實(shí)測可縮短10%-15%擴(kuò)增時(shí)間），同時(shí)減少溫度梯度差異，提高擴(kuò)增一致性。

3. FLEX-FREE結(jié)構(gòu)，操作更便捷：連排管體通過彈性連接固定，既避免運(yùn)輸脫落，又能單手掰取單管，無需剪刀或暴力拆分，減少耗材浪費(fèi)，尤其適合高通量實(shí)驗(yàn)場景。

4. 透明平蓋，觀察與密封兼顧：平蓋材質(zhì)高透光，可直接觀察管內(nèi)反應(yīng)液體積及顏色變化；同時(shí)平蓋與管體緊密貼合，經(jīng)壓力測試（500g壓力）無液體泄漏，有效防止氣溶膠污染，適配自動(dòng)化移液設(shè)備。

5. 全場景適配，兼容性強(qiáng)：無論是常規(guī)PCR、qPCR、逆轉(zhuǎn)錄PCR，還是基因測序、克隆驗(yàn)證，1402-4700八聯(lián)排管均表現(xiàn)穩(wěn)定，是實(shí)驗(yàn)室日常PCR實(shí)驗(yàn)的“萬能搭檔"。

常見疑問與解答

Q1：產(chǎn)品真的“無核酸酶"嗎？如何驗(yàn)證？

A：是的。1402-4700八聯(lián)排管采用伽馬射線輻照滅菌聯(lián)合無核酸酶處理工藝，出廠前經(jīng)第三方檢測（如Taq酶消化實(shí)驗(yàn)），確認(rèn)無DNase/RNase殘留，可提供完整質(zhì)檢報(bào)告。

Q2：薄壁設(shè)計(jì)會(huì)不會(huì)容易破裂？

A：不會(huì)。管體采用高韌性醫(yī)療級(jí)PP材質(zhì)，雖壁厚僅0.15mm，但抗沖擊性強(qiáng)（經(jīng)-20℃低溫脆化測試無破裂），可承受121℃高壓滅菌（15分鐘）及反復(fù)熱循環(huán)，耐用性優(yōu)于普通厚壁管。

Q3：透明平蓋的密封性如何？會(huì)不會(huì)漏液？

A：平蓋與管體通過模壓工藝緊密結(jié)合，經(jīng)壓力測試（500g靜置30分鐘）無液體滲漏，且表面光滑無凹陷，可有效防止氣溶膠污染，適配移液槍、自動(dòng)化工作站等設(shè)備。

Q4：是否適用于所有品牌PCR儀？

A：是的。管體尺寸嚴(yán)格符合國際標(biāo)準(zhǔn)（直徑12mm，高度47mm），兼容主流品牌熱循環(huán)儀（如ABI、Bio-Rad、Eppendorf、Roche等），無需額外調(diào)整孔板適配器。

Q5：可以高溫滅菌嗎？滅菌后性能會(huì)變化嗎？

A：支持121℃高壓滅菌（15分鐘），滅菌后管體無變形、無材質(zhì)老化，無核酸酶活性殘留，不影響后續(xù)實(shí)驗(yàn)性能。

Q6：與普通八聯(lián)排管相比，優(yōu)勢在哪里？

A：普通八聯(lián)排管可能存在核酸酶污染風(fēng)險(xiǎn)（未做去酶處理），且多為厚壁設(shè)計(jì)（影響擴(kuò)增效率）；此外，普通連蓋管拆條時(shí)易斷裂，浪費(fèi)耗材。1402-4700八聯(lián)排管通過無酶處理、薄壁設(shè)計(jì)及FLEX-FREE結(jié)構(gòu)，綜合解決了這些問題。

Q7：包裝規(guī)格是多少？

A：每盒含100條八聯(lián)排管（即800個(gè)單管），每條獨(dú)立塑封包裝，方便取用和存儲(chǔ)，避免交叉污染。

Q8：存儲(chǔ)條件有什么要求？

A：未開封時(shí)建議常溫（15-30℃）干燥保存，避免陽光直射；開封后需密封保存，建議6個(gè)月內(nèi)用完，以保證最佳性能。

Q9：保質(zhì)期多久？

A：未開封狀態(tài)下保質(zhì)期為2年（自生產(chǎn)日期起算），產(chǎn)品包裝標(biāo)注具體生產(chǎn)日期及有效期。

Q10：是否兼容qPCR染料（如SYBR Green）？

A：是的。管體材質(zhì)高透明、無熒光淬滅，可清晰觀察qPCR反應(yīng)體系的熒光信號(hào)，適用于SYBR Green、TaqMan等主流熒光染料。

（注：本文內(nèi)容基于品牌公開資料及行業(yè)常規(guī)信息整理，具體以品牌信息文檔為準(zhǔn)。）

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貨號(hào)	品名	規(guī)格	品牌
1402-9200	TempPlate® Semi-Skirted 96-Well PCR Plate, Straight Skirt, 0.2 mL	5 sleeves of 2 (10)	USAscientific
1402-2900	TEMPASSURE 0.2 ML PCR PULL-APART 8-TUBE STRIPS, ATTACHED INDIVIDUAL DOME CAPS	120 strips of 8 (960 tubes/caps) per box	USAscientific
1402-2400	TEMPASSURE 0.2 ML PCR PULL-APART TUBE STRIPS WITH DOME CAP STRIPS	80 strips of 12 tubes (960 tubes)	USAscientific
2932-0110	Cold Storage Foil	50 sheets	USAscientific
1180-1840	TipOne® RPT Filter Tips	10 racks of 96 (960)	USAscientific
1181-3810	10ul TipOne RPT filter tip	Case of 960	USAscientific
1182-1830	FILTER TIP ST 1000UL XL TIPONE	10 racks of 96 (960)	USAscientific
1180-8810	TipOne RPT Filter Tips	10 racks of 96 (960)	USAscientific
1183-1810	20UL TIPONE RPT FLTR TIP 960PK	Pack of 960	USAscientific
1180-1810	RPT 1-20UL FILTER TIP 960/CS	Case of 960	USAscientific
1412-0000	1.2 mL Tube, Individual, Non-Automation Rack	1000/Pack	USAscientific
1180-3810	TIPONE® RPT FILTER TIPS	Case of 960	USAscientific
91886775	USA Scientific Inc Titer-Tops film, pierceable, sterile, 50/bx	Pack of 50	USAscientific
1884-2410	0.2 mL deep 384-well PlateOne polypropylene plate, square wells, natural, individually wrapped	40/pack	USAscientific
1402-2300	TEMPASSURE PCR FLEX-FREE8-TUBE STRIPS，ATTACHEDINDIVIDUAL OPTICAL CAPS	0.1ml	USAscientific
2381-5048	81-place color-coded box, clear lid, mixed colors	Pack of 5	USAscientific
1111-3240	TIPONE® PIPETTE TIP STACKS，10 µL natural graduated	10 stacks (9600 tips)	USAscientific
1111-0200	TIPONE PIPETTE TIPSTACKS ，200 µL natural	1 stack (960 tips)	USAscientific
1111-3200	TIPONE PIPETTE TIPSTACKS， 10 µL natural graduated	1 stack (960 tips)	USAscientific
1212-8000	1.1 ml tubes in strips of 8, natural, polypropylene, bulk, 120 strips （960 tubes）	960 tubes	USAscientific
1412-8410	1.2 ML DILUTION TUBES IN STRIPS OF 8	RACK (10 RACKS OF 96)	USAscientific
1415-2500	1.5 ML COPOLYMERMICROCENTRIFUGE TUBE	500/bag	USAscientific
2920-0000	Microplate Adhesive Film	100 sheets/box	USAscientific
2344-1000	TOTALLY TUBUL AR RACK SYSTEM	1set	USAscientific
9124-0100	CoolSafe Block 96 wells Chamber	0.2 mL/96-well PCR	USAscientific
1420-9701	SCREW CAP MICROCENTRIFUGE TUBES, SELF-STANDING	ea	USAscientific
1183-1740	TIPONE RPT FILTER TIP REFILLS	100ul	USAscientific
1412-1000	1.2 ml tube	1000 個(gè)/包	USAscientific
1402-4708	TEMPASSURE PCR FLEX-FREE 8-TUBE STRIPS, ATTACHED INDIVIDUAL OPTICAL CAPS	ea	USAscientific
2380-1008	80-place tube rack, mixed colors, PP, 7 racks	7 racks	USAscientific
9187-1708	USA Scientific Inc Cryo-Babies, 1.28 inch x 0.5 inch, assorted colors, on sheets	85/sheet, 1700/box	USAscientific
1122-1760	TipOne 1000 μl XL graduated, refill, 40 cassettes of 96 tips	96tips	USAscientific
1120-8780	TipOne 200 μl graduated filter tip refill 8-unit case, sterile, 7680 tips	7680tips	USAscientific
1120-1760	TipOne 100 μl filter tip refill 8-unit case, sterile, 7680 tips	7680tips	USAscientific
1120-1880	TipOne 20 μl filter tip 8-unit case, sterile, 7680 tips	7680tips	USAscientific
1121-3880	TipOne 10 μl filter tip 8-unit case, sterile, 7680 tips	7680tips	USAscientific
1420-9710	2.0 Self standing Microcentrifuge tube, 500/pack Sterile	500/pack	USAscientific
1075-0110	5mL serological pipette	200.pack	USAscientific
1420-9410	TETHERED SCREW CAP MICROCENTRIFUGE TUBES, SELF-STANDING	100/pack	USAscientific
9123-6100	Breathe-Easy film, sterile	ea	USAscientific
2998-6550	LIGHT REFLECTING FILM	50/box	USAscientific
1405-4707	0.5 ml screw cap tube amber	500/pack	USAscientific
1420-9709	2.0 ml self-standing screw cap tube, black	500/pack	USAscientific
1475-0511	15mL Conical Tubes	500 pack	USAscientific
1071-0810	10 ml serological pipet, sterile	100pack	USAscientific
1620-2700	SEAL-RITE® 2.0 ML MICROCENTRIFUGE TUBES	500/pack	USAscientific
1500-1211	50 ml conical screw cap tubes	25 bags of 20	USAscientific
1420-9700	2.0 ml self-standing microcentrifuge tube, graduated, natural with natural cap	500/pack	USAscientific
1111-2820	TipOne® Natural, Graduated, Racked Pipette Tips, 101-1001ul	960 tips	USAscientific
1402-3900	TEMPASSURE PCR PULL-APART 8-TUBE STRIPS, ATTACHED INDIVIDUAL OPTICAL CAPS	120 strips of 8 (960 tubes/caps) per box	USAscientific
9138-2750	Direct Thermal Cryo-Tags®，SIZE: 1.5" × 0.5"，COLOR: White	ea	USAscientific
2920-0010	MICROPLATE SEALING FILM Standard cut, sterile	100 sheets	USAscientific
9187-2016	TOUGH-TAGS LABELS ON SHEETS	SIZE: 1.28" × 0.5" White	USAscientific
9185-2000	TOUGH-SPOTS LABELS ON SHEETS	SIZE: 1/2" diameter，White	USAscientific
1111-2021	1000 µL blue graduated	1000 tips/bag	USAscientific
2530-1102	Thermal-Lok 2-Position Dry Heat Bath, Includes 2 Blocks	2 Blocks	USAscientific
1833-9600	PLATEONE:emoji: 96-WELL POLYPROPYLENE PLATE	Conical (V) Non-sterile	USAscientific
1306-2510	CHANNELMATE RESERVOIR SYSTEM	25ml	USAscientific
2939-6100	SINGLE TAB SEALING FOIL, INSET CUT	100/box	USAscientific
1420-9799	2.0 ml self-standing screw cap tube, mixed color caps	500/pack	USAscientific
1011-8000	200 μL LARGE ORIFICE TIP	1000 tips/bag	USAscientific
1111-0240	TIPONE PIPETTE TIP STACKS (200 uL natural)	10 stacks (9600 tips)	USAscientific
3823-3412（ Non-sterile）	12-WELL V-BOTTOM RESERVOIR；3ml	Non-sterile 50/pack	USAscientific
1110-1240	1-200ul tip racks (5 stacks, no filter) Natural； 10 stacks (9600 tips)	10*5 stacks (9600 tips)	USAscientific
1111-0246	1-200ul tip racks (5 stacks, no filter)Yellow； 10 stacks (9600 tips)	10*5 stacks (9600 tips)	USAscientific
3823-3512	12-WELL V-BOTTOM RESERVOIR	20/pack	USAscientific
3823-3412	12-WELL V-BOTTOM RESERVOIR（）	20/pack	USAscientific
1061-8150	12-CHANNEL RESERVOIR FOR AUTOMATION	5/pack	USAscientific
1615-5500	SEAL-RITE® 1.5 ML MICROCENTRIFUGE TUBES	500/bag	USAscientific
1111-0206	TIPONE PIPETTE TIPS IN RACKS	10 racks of 96 (960)	USAscientific
1111-2821	TIPONE PIPETTE TIPS IN RACKS	10 racks of 96 (960)	USAscientific
1126-7810	TIPONE FILTER TIPS	10PACKS OF 96(960)	USAscientific
1120-8810	TIPONE FILTER TIPS	10PACKS OF 96(960)	USAscientific
1121-3810	TIPONE FILTER TIPS	10uL	USAscientific
1022-0600	200 μL GEL-SAVER® ROUND GEL LOADING TIP	5 racks of 204	USAscientific
1011-8410	200 μL LARGE ORIFICE TIP sterile	10 racks of 96 (960)	USAscientific
2512-0000	THERMAL-LOK BLOCK FOR 15 ML TUBES	15 ML TUBES	USAscientific
2510-1102	Thermal-Lok 2-Position Dry Heat Bath (Without Blocks)	ea	USAscientific
2923-0110	TempPlate® Sealing Foil	50sheets/box	USAscientific
2920-1010	MICROPOROUS FILM	50/box	USAscientific
2923-5000	NAL-96 PIERCEABLE FILM	50 sheets/box	USAscientific
1301-1212	Twelve well reservoir with lid, individually wrapped, sterile, 20/pack.	20/pack	USAscientific
1111-1200	TIPONE PIPETTE TIP STACKS	960 tips	USAscientific
9152-2200	LABELING TAPE, 0.75IN WIDE	2160 inch	USAscientific
1111-2721	TIPONE PIPETTE TIP REFILLS，1000 μL blue graduated	10 wafers of 96 (960)	USAscientific
1111-0706	TIPONE PIPETTE TIP REFILLS，200 μL yellow	10 wafers of 96 (960)	USAscientific
1111-0506	TIPONE PIPETTE TIP STARTER SYSTEMS	200 uL yellow	USAscientific
1111-2521	TIPONE PIPETTE TIP STARTER SYSTEMS	1000 uL blue graduated	USAscientific
1110-3700	TIPONE PIPETTE TIP REFILLS，10 μL XL natural graduated	10 wafers of 96 (960)	USAscientific
1110-3500	TipOne Pipette Tip Starter Systems	10 uL XL natural graduated	USAscientific
1111-3500	TipOne Pipette Tip Starter Systems	10 uL natural graduated	USAscientific
7400-2401	Glass disruptor beads, 0.1 mm diameter, Scientific Industries SI-BG01	each	USAscientific
1420-9707	SCREW CAP MICROCENTRIFUGE TUBES, SELF-STANDING, AMBER（2.0 ml棕色tube）	500 tubes	USAscientific
1605-0002	Green SEAL-RITE® 0.5 ML MICROCENTRIFUGE TUBES	1000/pack	USAscientific
1605-0006	Yellow SEAL-RITE® 0.5 ML MICROCENTRIFUGE TUBES	1000/pack	USAscientific
1605-0007	Amber SEAL-RITE® 0.5 ML MICROCENTRIFUGE TUBES	1000/pack	USAscientific
1405-2600	0.5 ml low adhesion microcentrifuge tube, natural	500/bag	USAscientific
1415-2600	1.5 ml low adhesion microcentrifuge tube, natural 無核酸酶非粘性1.5ml試管	bag of 500	USAscientific
1405-9707	0.5 ml self-standing screw cap tube amber	500/pack	USAscientific
1402-4700	0.2 ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八聯(lián)排無核酸酶薄壁PCR管(0.2ml)	盒	USAscientific